Highly stable and reusable immobilized formate dehydrogenases: Promising biocatalysts for in situ regeneration of NADH

• Barış Binay,
• Dilek Alagöz,
• Deniz Yildirim,
• Ayhan Çelik and
• S. Seyhan Tükel

Beilstein J. Org. Chem. 2016, 12, 271–277, doi:10.3762/bjoc.12.29

• formate dehydrogenase (FDH) preparations which can be used as effective biocatalysts along with functional oxidoreductases, in which in situ regeneration of NADH is required. For this purpose, Candida methylica FDH was covalently immobilized onto Immobead 150 support (FDHI150), Immobead 150 support

• , FDHIGLU and FDHIALD offer feasible potentials for in situ regeneration of NADH. Keywords: biocatalysis; Candida methylica; formate dehydrogenase; Immobead 150; regeneration of NADH; stabilization; Introduction Dehydrogenases are one of the most promising enzymes in biocatalysis since these enzymes have

• decrease operational costs. NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) catalyzes oxidation of formate to carbon dioxide (CO2) [6]. FDH is industrially used as coenzyme for the regeneration of NADH [7][8], as sensor for the determination of formic acid [9], and as catalyst for the production of

An integrated photocatalytic/enzymatic system for the reduction of CO₂ to methanol in bioglycerol–water

• Michele Aresta,
• Angela Dibenedetto,
• Tomasz Baran,
• Antonella Angelini,
• Przemysław Łabuz and
• Wojciech Macyk

Beilstein J. Org. Chem. 2014, 10, 2556–2565, doi:10.3762/bjoc.10.267

• to NAD+. However, the production of one mole of CH3OH from CO2 requires the consumption of three moles of NADH (Figure 1). Therefore, the regeneration of NADH is necessary for practical application of the described process. In nature, the endergonic process of NAD+ reduction to NADH is performed with

• conditions, resulting in CH3OH at room temperature. The regeneration of NADH was attempted using both chemical and photochemical techniques. The former affects the enzymes that are quickly deactivated, while the latter techniques are more interesting and produce up to a few mol of methanol per mol of NADH

• was evidenced by measuring the amount of photogenerated 1,4-NADH using water or water/glycerol as electron donor. Figure 3b illustrates the results of the photocatalytic regeneration of NADH in presence of various materials after 6 hours of irradiation. Interestingly, for [CrF5(H2O)]2−@TiO2 the yield

Coupled chemo(enzymatic) reactions in continuous flow

• Ruslan Yuryev,
• Simon Strompen and
• Andreas Liese

Beilstein J. Org. Chem. 2011, 7, 1449–1467, doi:10.3762/bjoc.7.169

• turnover number increased by a factor of 3.4 compared to a reactor without cofactor retention. The regeneration of NADH was achieved in a coupled biocatalytic oxidation of formate (2). Hecke and coworkers described the production of lactobionic acid (13) from lactose (12) in a coupled two-enzyme reaction